Part:BBa_K4850013

Green Fluorescence Protein (GFP)

Green fluorescence protein (GFP) is an important reporter molecule that would emit bright green light when excited. It has been widely used as a reporter gene in various experiments as it allows the visualization of the gene expression in vivo.

Usage and Biology

The NUS-Singapore 2023 iGEM team incorporated the GFP sequence into two distinct plasmids: one containing the sacB gene and the other featuring a diametric selection marker. In both cases, the GFP sequences are physically linked to the sacB gene/the Kanamycin resistance gene by a protein linker A(EAAAK)₂A (BBa_K4850006), resulting in the production of a fusion protein. For more information, please visit:

• BBa_K4850000: sacB Gene (Basic Part)
• BBa_K4850008: GFP-sacB Negative Selection Marker (Composite Part)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 644