![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
Part:BBa_K4850013
Designed by: Chai Chin Wei Group: iGEM23_NUS-Singapore (2023-10-11)
Green Fluorescence Protein (GFP)
Green fluorescence protein (GFP) is an important reporter molecule that would emit bright green light when excited. It has been widely used as a reporter gene in various experiments as it allows the visualization of the gene expression in vivo.
Usage and Biology
The NUS-Singapore 2023 iGEM team incorporated the GFP sequence into two distinct plasmids: one containing the sacB gene and the other featuring a diametric selection marker. In both cases, the GFP sequences are physically linked to the sacB gene/the Kanamycin resistance gene by a protein linker A(EAAAK)2A (BBa_K4850006), resulting in the production of a fusion protein. For more information, please visit:
- BBa_K4850000: sacB Gene (Basic Part)
- BBa_K4850008: GFP-sacB Negative Selection Marker (Composite Part)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
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Categories
Parameters
emission | 528nm |
excitation | 485nm |